TITULO FUENTE ORIGINAL: Oral self-administration of buprenorphine in the diet for analgesia in mice
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AUTORES: Molina-Cimadevila MJ, Segura S, Merino C, Ruiz-Reig N, Andrés B, de Madaria E.
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REFERENCIA: 48(3):216-224
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DOI: 10.1177/0023677214532454
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RESUMEN ORIGINAL:
Postsurgical oral self-administration of analgesics in rodents is an interesting technique of providing analgesia, avoiding the negative effects of manipulation. Several strategies, using gelatin or nutella, have already been described. However, rodents require some habituation period to reach a good intake because of their neophobic behavior. The current study aimed to explore whether... + Leer más Postsurgical oral self-administration of analgesics in rodents is an interesting technique of providing analgesia, avoiding the negative effects of manipulation. Several strategies, using gelatin or nutella, have already been described. However, rodents require some habituation period to reach a good intake because of their neophobic behavior. The current study aimed to explore whether buprenorphine when mixed with an extruded diet offers a potential treatment option in the pain management of mice using a triple approach: by measuring the spontaneous intake in healthy animals; by using the hot-plate test; and finally by assessing the drug's ability to provide postoperative analgesia in a surgical intervention of moderate severity (intra-utero electroporation). Mice consumed during 20 hours, similar amounts of extruded diet alone, mixed with glucosaline, and mixed with buprenorphine (0.03 mg per pellet) or meloxicam (0.25 mg per pellet) both of which were diluted in glucosaline, showing that no neophobia was associated with these administrations. Relative increase from baseline latency (% maximal possible effect) in the hot-plate test at 20 h of administration was significantly higher for oral buprenorphine in diet 0.03 mg/pellet, and diet 0.15 mg/pellet, compared with placebo and no differences were found between those oral administrations and subcutaneous buprenorphine 0.1 mg/kg measured 3 h later. The treatment was also effective in attenuating the reductions in food consumption and body weight that occur after surgery. These data suggest that providing buprenorphine with the diet is a feasible and effective way of self-administration of analgesia in mice and does not cause neophobia and may easily contribute to the refinement of surgical procedures. - Leer menos
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COMENTARIO DE Ignacio, Álvarez Gómez de Segura
La administración continuada de analgésicos opioides suele ser un problema logístico y de bienestar para los animales. De forma idónea la administración parenteral debería restringirse al mínimo, reduciendo la manipulación de los animales, así como someterles a procedimientos estresantes como la inyección. Por otro lado, la... + Leer más La administración continuada de analgésicos opioides suele ser un problema logístico y de bienestar para los animales. De forma idónea la administración parenteral debería restringirse al mínimo, reduciendo la manipulación de los animales, así como someterles a procedimientos estresantes como la inyección. Por otro lado, la disponibilidad de opiáceos formulados para un efecto prolongado es escasa o no está disponible. Por ejemplo, aunque existe en el mercado una formulación de buprenorfina cuyo efecto se prolonga varios días, la misma no está disponible en todos los países. En este artículo se plantea una fórmula sencilla, al alcance de cualquier laboratorio, de administración de analgésicos incorporando el fármaco a la dieta o pienso. De este modo, los autores incorporan en cada pellet 0,03 mg de buprenorfina, pero pudiendo incorporar otros analgésicos como el meloxicam. Dicha combinación no reduce la ingesta de comida de los ratones y, cuando se valoró la buprenorfina, se observó que produjo una analgesia similar a la observada tras su administración subcutánea. - Leer menos
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