REVISTA

La bupivacaína induce la apoptosis a través de vías de caspasas en las células tumorales mamarias caninas

Descripción: Nuestros datos sugieren que la apoptosis inducida por bupivacaína se produce a través de vías apoptóticas tanto dependientes de caspasa como independientes de caspasa en celulas tumorales mamarias caninas

TITULO FUENTE ORIGINAL:

Bupivacaine induces apoptosis through caspase-dependent and -independent pathways in canine mammary tumor cells

AUTORES:

Chiu YS, Cheng YH, Lin SW, Chang TS, Liou CJ, Lai YS

REVISTA ABREV.:

Res Vet Sci

AÑO:

2015

REFERENCIA:

100:232-8

DOI:

10.1016/j.rvsc.2015.03.024

RESUMEN ORIGINAL:

Local anesthetics have been reported to induce apoptosis in various cell lines. In this study, we showed that bupivacaine also induced apoptosis in DTK-SME cells, a vimentin(+)/AE1(+)/CK7(+)/HSP27(+), tumorigenic, immortalized, canine mammary tumor cell line. Bupivacaine induced apoptosis in DTK-SME cells in a time- and concentration-dependent manner. Apoptosis-associated morphological... + Leer más

Local anesthetics have been reported to induce apoptosis in various cell lines. In this study, we showed that bupivacaine also induced apoptosis in DTK-SME cells, a vimentin(+)/AE1(+)/CK7(+)/HSP27(+), tumorigenic, immortalized, canine mammary tumor cell line. Bupivacaine induced apoptosis in DTK-SME cells in a time- and concentration-dependent manner. Apoptosis-associated morphological changes, including cell shrinkage and rounding, chromatin condensation, and formation of apoptotic bodies, were observed in the bupivacaine-treated DTK-SME cells. Apoptosis was further confirmed with annexin V staining, TUNEL staining, and DNA laddering assays. At the molecular level, the activation of caspases-3, -8, and -9 corresponded well to the degree of DNA fragmentation triggered by bupivacaine. We also demonstrated that the pan-caspase inhibitor, z-VAD-fmk, only partially inhibited the apoptosis induced by bupivacaine. Moreover, treated cells increased expression of endonuclease G, a death effector that acts independently of caspases. Our data suggested that bupivacaine-induced apoptosis occurs through both caspase-dependent and caspase-independent apoptotic pathways

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