Efecto de los glucocorticoides intratecales sobre el receptor central de glucocorticoides en un modelo de ligadura del nervio de rata

REVISTA

Descripción: No disminuyó la hipersensibilidad mecánica inducida por la ligadura

TITULO FUENTE ORIGINAL:

Effect of intrathecal glucocorticoids on the central glucocorticoid receptor in a rat nerve ligation model

AUTORES:

Mienke Rijsdijk, Nilesh M Agalave, Albert J M van Wijck, Cornelis J Kalkman, Roshni Ramachandran, Azar Baharpoor, Camilla I Svensson, Tony L Yaksh

REVISTA ABREV.:

Scand J Pain

AÑO:

2017

REFERENCIA:

16:1-9

DOI:

10.1016/j.sjpain.2016.12.008

RESUMEN ORIGINAL:

Background and aims: Despite widespread use, the efficacy of neuraxial glucocorticoids for neuropathic pain is subject to debate. Since most glucocorticoid actions are mediated through its receptor, we explored the effects of intrathecal methylprednisolone acetate (MPA) on total glucocorticoid receptor (tGR) levels and activation of the glucocorticoid receptor (phosphorylated state=pGR) within... + Leer más

Background and aims: Despite widespread use, the efficacy of neuraxial glucocorticoids for neuropathic pain is subject to debate. Since most glucocorticoid actions are mediated through its receptor, we explored the effects of intrathecal methylprednisolone acetate (MPA) on total glucocorticoid receptor (tGR) levels and activation of the glucocorticoid receptor (phosphorylated state=pGR) within the spinal dorsal horn (SDH) and dorsal root ganglion (DRG) in a spinal nerve ligation (SNL) model in rats.

Methods: Rats received unilateral ligation of the L5/L6 spinal nerves and were treated with two intrathecal doses of either 400μg MPA or 0.9% saline with a 72-h interval. Plantar tactile thresholds were measured over time. Seven days after drug treatment, DRG and SDH were harvested to assess tGR and pGR levels using immunohistochemistry and qPCR.

Results: Allodynia, defined by lowered tactile withdrawal thresholds after SNL, was unaltered by intrathecal MPA. In saline controls, mRNA levels of tGR did not change after SNL in the DRGs or SDH. tGR and pGR protein levels in the SDH however, significantly increased on the ipsilateral side of SNL compared to the contralateral side and to naïve tissue. When treating rats with MPA, tGR mRNA levels were significantly reduced in the SDH compared to saline controls. tGR and pGR protein levels, however were not significantly lower compared to saline controls.

Conclusions: In intrathecal MPA treated rats, tGR mRNA levels decreased after SNL. However this did not result in lower tGR and pGR protein levels compared to saline controls, and did not decrease ligation-induced mechanical hypersensitivity.

Implications: Intrathecal MPA treatment after SNL did not result in lower tGR and pGR levels within the SDH and DRG compared to saline controls. In present study we did not differentiate between the various isoforms of the GR which might clarify this finding

- Leer menos